Hotspot

accD

LSC · 52170-54051 · 1882 bp

Back to Evolvulus

Pi 0.00725

MarkerSeek score 66.60

Primer available yes

Label accD

Feature Metadata

label_name: accD
feature_type: gene
region: LSC
length_bp: 1882
variable_sites: 13
pi: 0.00725
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 66.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD_p1	CATGCAAATAGGGGGCAAGA	TGCTTTCTTAGATCTTCTGTTCCT	50.0	37.5	58.2	57.6	1956-2029 bp	1.0	47.4
accD_p2	TCATGCAAATAGGGGGCAAG	TGCTTTCTTAGATCTTCTGTTCCT	50.0	37.5	58.2	57.6	1957-2030 bp	1.0	47.4
accD_p3	TTCATGCAAATAGGGGGCAA	TGCTTTCTTAGATCTTCTGTTCCT	45.0	37.5	57.8	57.6	1958-2031 bp	1.0	47.2
accD_p4	TTTCATGCAAATAGGGGGCA	TGCTTTCTTAGATCTTCTGTTCCT	45.0	37.5	57.8	57.6	1959-2032 bp	1.0	47.2
accD_p5	TGCAAATAGGGGGCAAGAAA	TGCTTTCTTAGATCTTCTGTTCCT	45.0	37.5	57.7	57.6	1954-2027 bp	1.0	46.6