Hotspot

matK-trnK-UUU

LSC · 155779-156548 · 770 bp

Back to Evolvulus

Pi 0.503

MarkerSeek score 83.50

Primer available yes

Label matK-trnK-UUU

Feature Metadata

label_name: matK-trnK-UUU
feature_type: igs
region: LSC
length_bp: 770
variable_sites: 368
pi: 0.503
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 83.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK-trnK-UUU_p1	CCACAATCATGAGCAAGTGC	AACGGTAGAGTACTCGGCTT	50.0	50.0	57.7	58.2	854-901 bp	1.0	88.5
matK-trnK-UUU_p2	CCACAATCATGAGCAAGTGC	ACGGTAGAGTACTCGGCTTT	50.0	50.0	57.7	58.2	853-900 bp	1.0	88.5
matK-trnK-UUU_p3	CCACAATCATGAGCAAGTGC	GGTGGAACTTCGGCCTAATC	50.0	55.0	57.7	58.3	1313-1619 bp	1.0	87.1
matK-trnK-UUU_p4	CCACAATCATGAGCAAGTGC	GTGGAACTTCGGCCTAATCA	50.0	50.0	57.7	57.6	1312-1618 bp	1.0	86.6
matK-trnK-UUU_p5	CCACAATCATGAGCAAGTGC	TGAGGGTTGCTAACTCAACG	50.0	50.0	57.7	57.8	917 bp	0.5	56.5