Hotspot

trnN-GUU-ycf1

IRb · 103802-107097 · 3296 bp

Back to Evolvulus

Pi 0.00534

MarkerSeek score 51.20

Primer available yes

Label trnN-GUU-ycf1

Feature Metadata

label_name: trnN-GUU-ycf1
feature_type: igs
region: IRb
length_bp: 3296
variable_sites: 17
pi: 0.00534
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 51.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-ycf1_p1	AAAGTCCCCCGATGGTCATA	TTAAGCCTTGCCGAAAATCG	50.0	45.0	58.1	57.1	646-687 bp	1.0	77.6
trnN-GUU-ycf1_p2	AAAGTCCCCCGATGGTCATA	ATCGTACCGAAAACAGACGA	50.0	45.0	58.1	57.0	613-654 bp	1.0	77.0
trnN-GUU-ycf1_p3	AAAGTCCCCCGATGGTCATA	TCGTACCGAAAACAGACGAT	50.0	45.0	58.1	57.0	612-653 bp	1.0	77.0
trnN-GUU-ycf1_p4	CCGAATTGCAACAACTGGTG	TTAAGCCTTGCCGAAAATCG	50.0	45.0	58.2	57.1	615-656 bp	1.0	76.8
trnN-GUU-ycf1_p5	CGAATTGCAACAACTGGTGG	TTAAGCCTTGCCGAAAATCG	50.0	45.0	58.2	57.1	614-655 bp	1.0	76.8