Hotspot

trnS-CGA-trnR-UCU

LSC · 4142-5006 · 865 bp

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Pi 0.0525
MarkerSeek score 83.70
Primer available yes
Label trnS-CGA-trnR-UCU

Feature Metadata

label_name
trnS-CGA-trnR-UCU
feature_type
igs
region
LSC
length_bp
865
variable_sites
44
pi
0.0525
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
83.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-CGA-trnR-UCU_p1 AGCCTTCCAAGCTAACGATG AGAAGACCTCTGTCCTATCCA 50.0 47.6 58.0 56.9 946-1000 bp 1.0 69.2
trnS-CGA-trnR-UCU_p2 CCTAGCCTTCCAAGCTAACG AGAAGACCTCTGTCCTATCCA 55.0 47.6 58.1 56.9 949-1003 bp 1.0 68.9
trnS-CGA-trnR-UCU_p3 AGCCTTCCAAGCTAACGATG AGGTTTAGAAGACCTCTGTCCT 50.0 45.5 58.0 57.9 952-1006 bp 1.0 68.5
trnS-CGA-trnR-UCU_p4 CCTAGCCTTCCAAGCTAACG AGGTTTAGAAGACCTCTGTCCT 55.0 45.5 58.1 57.9 955-1009 bp 1.0 68.2
trnS-CGA-trnR-UCU_p5 CCCTAGCCTTCCAAGCTAAC AGAAGACCTCTGTCCTATCCA 55.0 47.6 57.7 56.9 950-1004 bp 1.0 66.7