primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnS-CGA-trnR-UCU_p1	trnS-CGA-trnR-UCU	1	AGCCTTCCAAGCTAACGATG	AGAAGACCTCTGTCCTATCCA	20	21	50.0	47.6	58.0	56.9	0.0	0.0	2.081443	4142	5006	946-1000	1.000000	69.2
trnS-CGA-trnR-UCU_p2	trnS-CGA-trnR-UCU	2	CCTAGCCTTCCAAGCTAACG	AGAAGACCTCTGTCCTATCCA	20	21	55.0	47.6	58.1	56.9	0.0	0.0	2.110280	4142	5006	949-1003	1.000000	68.9
trnS-CGA-trnR-UCU_p3	trnS-CGA-trnR-UCU	3	AGCCTTCCAAGCTAACGATG	AGGTTTAGAAGACCTCTGTCCT	20	22	50.0	45.5	58.0	57.9	0.0	0.0	2.164771	4142	5006	952-1006	1.000000	68.5
trnS-CGA-trnR-UCU_p4	trnS-CGA-trnR-UCU	4	CCTAGCCTTCCAAGCTAACG	AGGTTTAGAAGACCTCTGTCCT	20	22	55.0	45.5	58.1	57.9	0.0	0.0	2.193608	4142	5006	955-1009	1.000000	68.2
trnS-CGA-trnR-UCU_p5	trnS-CGA-trnR-UCU	5	CCCTAGCCTTCCAAGCTAAC	AGAAGACCTCTGTCCTATCCA	20	21	55.0	47.6	57.7	56.9	0.0	0.0	2.385293	4142	5006	950-1004	1.000000	66.7
accD_p1	accD	1	CATGCAAATAGGGGGCAAGA	TGCTTTCTTAGATCTTCTGTTCCT	20	24	50.0	37.5	58.2	57.6	0.0	0.0	4.586749	52170	54051	1956-2029	1.000000	47.4
accD_p2	accD	2	TCATGCAAATAGGGGGCAAG	TGCTTTCTTAGATCTTCTGTTCCT	20	24	50.0	37.5	58.2	57.6	0.0	0.0	4.586749	52170	54051	1957-2030	1.000000	47.4
accD_p3	accD	3	TTCATGCAAATAGGGGGCAA	TGCTTTCTTAGATCTTCTGTTCCT	20	24	45.0	37.5	57.8	57.6	0.0	0.0	4.610456	52170	54051	1958-2031	1.000000	47.2
accD_p4	accD	4	TTTCATGCAAATAGGGGGCA	TGCTTTCTTAGATCTTCTGTTCCT	20	24	45.0	37.5	57.8	57.6	0.0	0.0	4.610456	52170	54051	1959-2032	1.000000	47.2
accD_p5	accD	5	TGCAAATAGGGGGCAAGAAA	TGCTTTCTTAGATCTTCTGTTCCT	20	24	45.0	37.5	57.7	57.6	0.0	0.0	4.683342	52170	54051	1954-2027	1.000000	46.6
clpP_p1	clpP	1	GAATTCGAAGTGCCATGCTA	CAAAACCCTACGTTTCCCCA	20	20	45.0	50.0	56.2	58.0	1.3	0.0	1.798315	66323	68523	2244-2412	1.000000	70.0
clpP_p2	clpP	2	GAATTCGAAGTGCCATGCTA	TCAAAACCCTACGTTTCCCC	20	20	45.0	50.0	56.2	57.7	1.3	0.0	2.057515	66323	68523	2245-2413	1.000000	67.9
clpP_p3	clpP	3	GAATTCGAAGTGCCATGCTAT	CAAAACCCTACGTTTCCCCA	21	20	42.9	50.0	56.7	58.0	1.3	0.0	2.266790	66323	68523	2244-2412	1.000000	66.2
clpP_p4	clpP	4	AATTCGAAGTGCCATGCTAT	CAAAACCCTACGTTTCCCCA	20	20	40.0	50.0	55.5	58.0	3.3	0.0	2.521553	66323	68523	2243-2411	1.000000	64.2
clpP_p5	clpP	5	ATTCGAAGTGCCATGCTATT	CAAAACCCTACGTTTCCCCA	20	20	40.0	50.0	55.5	58.0	3.3	0.0	2.521553	66323	68523	2242-2410	1.000000	64.2
trnI-CAU-ycf2_p1	trnI-CAU-ycf2	1	ATTGAACCTACGACTTCGCC	TGAATTCAATTGGGTCCACGA	20	21	50.0	42.9	58.0	57.9	0.0	5.4	1.153524	82671	83203	635-650	1.000000	73.1
trnI-CAU-ycf2_p2	trnI-CAU-ycf2	2	GAACCTACGACTTCGCCAAT	TGAATTCAATTGGGTCCACGA	20	21	50.0	42.9	58.0	57.9	0.0	5.4	1.153524	82671	83203	632-647	1.000000	73.1
trnI-CAU-ycf2_p3	trnI-CAU-ycf2	3	ATTGAACCTACGACTTCGCC	TCAATTGGGTCCACGAATCT	20	20	50.0	45.0	58.0	56.8	0.0	5.4	1.177783	82671	83203	630-645	1.000000	73.0
trnI-CAU-ycf2_p4	trnI-CAU-ycf2	4	GAACCTACGACTTCGCCAAT	TCAATTGGGTCCACGAATCT	20	20	50.0	45.0	58.0	56.8	0.0	5.4	1.177783	82671	83203	627-642	1.000000	73.0
trnI-CAU-ycf2_p5	trnI-CAU-ycf2	5	GATGGTGTGCATCCAGTAGG	TGAATTCAATTGGGTCCACGA	20	21	55.0	42.9	58.0	57.9	16.5	5.4	1.188512	82671	83203	656-671	1.000000	72.8
ycf2-trnL-CAA_p1	ycf2-trnL-CAA	1	ATGGCTTTTCCCGGATGAAA	AAATGGTAGACACGCGAGAC	20	20	45.0	50.0	57.8	58.0	0.7	15.9	0.237960	89552	89712	270-270	1.000000	79.3
ycf2-trnL-CAA_p2	ycf2-trnL-CAA	2	TGGCTTTTCCCGGATGAAAT	AAATGGTAGACACGCGAGAC	20	20	45.0	50.0	57.8	58.0	0.7	15.9	0.237960	89552	89712	269-269	1.000000	79.3
ycf2-trnL-CAA_p3	ycf2-trnL-CAA	3	ATGGCTTTTCCCGGATGAAA	TGAAATGGTAGACACGCGAG	20	20	45.0	50.0	57.8	58.0	0.7	15.9	0.239330	89552	89712	272-272	1.000000	79.3
ycf2-trnL-CAA_p4	ycf2-trnL-CAA	4	TGGCTTTTCCCGGATGAAAT	TGAAATGGTAGACACGCGAG	20	20	45.0	50.0	57.8	58.0	0.7	15.9	0.239330	89552	89712	271-271	1.000000	79.3
ycf2-trnL-CAA_p5	ycf2-trnL-CAA	5	AAGATGGCTTTTCCCGGATG	AAATGGTAGACACGCGAGAC	20	20	50.0	50.0	58.2	58.0	0.7	15.9	0.240071	89552	89712	273-273	1.000000	79.3
trnN-GUU-ycf1_p1	trnN-GUU-ycf1	1	AAAGTCCCCCGATGGTCATA	TTAAGCCTTGCCGAAAATCG	20	20	50.0	45.0	58.1	57.1	0.0	0.0	1.024022	106724	106843	646-687	1.000000	77.6
trnN-GUU-ycf1_p2	trnN-GUU-ycf1	2	AAAGTCCCCCGATGGTCATA	ATCGTACCGAAAACAGACGA	20	20	50.0	45.0	58.1	57.0	0.0	0.0	1.107669	106724	106843	613-654	1.000000	77.0
trnN-GUU-ycf1_p3	trnN-GUU-ycf1	3	AAAGTCCCCCGATGGTCATA	TCGTACCGAAAACAGACGAT	20	20	50.0	45.0	58.1	57.0	0.0	0.0	1.107669	106724	106843	612-653	1.000000	77.0
trnN-GUU-ycf1_p4	trnN-GUU-ycf1	4	CCGAATTGCAACAACTGGTG	TTAAGCCTTGCCGAAAATCG	20	20	50.0	45.0	58.2	57.1	0.0	0.0	1.142086	106724	106843	615-656	1.000000	76.8
trnN-GUU-ycf1_p5	trnN-GUU-ycf1	5	CGAATTGCAACAACTGGTGG	TTAAGCCTTGCCGAAAATCG	20	20	50.0	45.0	58.2	57.1	0.0	0.0	1.142086	106724	106843	614-655	1.000000	76.8
trnL-CAA-ycf2_p1	trnL-CAA-ycf2	1	AAATGGTAGACACGCGAGAC	ATGGCTTTTCCCGGATGAAA	20	20	50.0	45.0	58.0	57.8	15.9	0.7	0.237960	147564	147724	270-270	1.000000	80.2
trnL-CAA-ycf2_p2	trnL-CAA-ycf2	2	AAATGGTAGACACGCGAGAC	TGGCTTTTCCCGGATGAAAT	20	20	50.0	45.0	58.0	57.8	15.9	0.7	0.237960	147564	147724	269-269	1.000000	80.2
trnL-CAA-ycf2_p3	trnL-CAA-ycf2	3	TGAAATGGTAGACACGCGAG	TGGCTTTTCCCGGATGAAAT	20	20	50.0	45.0	58.0	57.8	15.9	0.7	0.239330	147564	147724	271-271	1.000000	80.2
trnL-CAA-ycf2_p4	trnL-CAA-ycf2	4	TGAAATGGTAGACACGCGAG	ATGGCTTTTCCCGGATGAAA	20	20	50.0	45.0	58.0	57.8	15.9	0.7	0.239330	147564	147724	272-272	1.000000	80.1
trnL-CAA-ycf2_p5	trnL-CAA-ycf2	5	AAATGGTAGACACGCGAGAC	AAGATGGCTTTTCCCGGATG	20	20	50.0	50.0	58.0	58.2	15.9	0.7	0.240071	147564	147724	273-273	1.000000	80.1
trnK-UUU_p1	trnK-UUU	1	ATCGTCAAATCTCGCTTGCT	TGGCTTTAAAAGGGACGCTT	20	20	45.0	45.0	58.0	58.0	0.0	0.0	0.026030	154161	154196	1038-1038	0.500000	59.8
trnK-UUU_p2	trnK-UUU	2	ATCGTCAAATCTCGCTTGCT	TTGGCTTTAAAAGGGACGCT	20	20	45.0	45.0	58.0	58.0	0.0	0.0	0.026030	154161	154196	1039-1039	0.500000	59.8
trnK-UUU_p3	trnK-UUU	3	GTTGAACCCTGGAAATTGCG	TGGCTTTAAAAGGGACGCTT	20	20	50.0	45.0	57.9	58.0	0.0	0.0	0.074867	154161	154196	1017-1017	0.500000	59.4
trnK-UUU_p4	trnK-UUU	4	GTTGAACCCTGGAAATTGCG	TTGGCTTTAAAAGGGACGCT	20	20	50.0	45.0	57.9	58.0	0.0	0.0	0.074867	154161	154196	1018-1018	0.500000	59.4
trnK-UUU_p5	trnK-UUU	5	ATCGTCAAATCTCGCTTGCT	CCAGCCATTCCCTTGACTTT	20	20	45.0	50.0	58.0	58.1	0.0	0.0	0.086868	154161	154196	921-921	0.500000	59.3
trnK-UUU_p1	trnK-UUU	1	TGAATTCAATTGGGTCCACGA	GGTGGAACTTCGGCCTAATC	21	20	42.9	55.0	57.9	58.3	5.4	0.0	1.482680	154161	156585	2969-2969	0.500000	48.1
trnK-UUU_p2	trnK-UUU	2	TCAATTGGGTCCACGAATCT	GGTGGAACTTCGGCCTAATC	20	20	45.0	55.0	56.8	58.3	5.4	0.0	1.506939	154161	156585	2964-2964	0.500000	47.9
trnK-UUU_p3	trnK-UUU	3	TGAATTCAATTGGGTCCACGA	GTGGAACTTCGGCCTAATCA	21	20	42.9	50.0	57.9	57.6	5.4	0.0	1.544143	154161	156585	2968-2968	0.500000	47.6
trnK-UUU_p4	trnK-UUU	4	TCAATTGGGTCCACGAATCT	GTGGAACTTCGGCCTAATCA	20	20	45.0	50.0	56.8	57.6	5.4	0.0	1.568402	154161	156585	2963-2963	0.500000	47.5
trnK-UUU_p5	trnK-UUU	5	TGTGAATGAAGGATCCCACTG	GGTGGAACTTCGGCCTAATC	21	20	47.6	55.0	57.6	58.3	8.6	0.0	1.690075	154161	156585	2988-2988	0.500000	46.5
matK_p1	matK	1	ATCGTCAAATCTCGCTTGCT	AAAGGGGGATTCGAAGGAGA	20	20	45.0	50.0	58.0	58.0	0.0	3.3	0.048891	154468	155778	2091-2091	0.500000	59.6
matK_p2	matK	2	ATCGTCAAATCTCGCTTGCT	GCAACGAGCTTTGGTTCTTC	20	20	45.0	50.0	58.0	58.0	0.0	0.5	0.053677	154468	155778	2392-2392	0.500000	59.6
matK_p3	matK	3	ATCGTCAAATCTCGCTTGCT	AGAGTTTGTAAGACCGCGAC	20	20	45.0	50.0	58.0	57.9	0.0	8.2	0.077476	154468	155778	2598-2598	0.500000	59.4
matK_p4	matK	4	ATCGTCAAATCTCGCTTGCT	GAGTTTGTAAGACCGCGACT	20	20	45.0	50.0	58.0	57.9	0.0	8.2	0.077476	154468	155778	2597-2597	0.500000	59.4
matK_p5	matK	5	ATCGTCAAATCTCGCTTGCT	TCTGCCTTTGGTCCAAATCC	20	20	45.0	50.0	58.0	58.1	0.0	17.4	0.092380	154468	155778	1941-1941	0.500000	59.3
matK-trnK-UUU_p1	matK-trnK-UUU	1	CCACAATCATGAGCAAGTGC	AACGGTAGAGTACTCGGCTT	20	20	50.0	50.0	57.7	58.2	0.1	17.1	0.441789	155779	156548	854-901	1.000000	88.5
matK-trnK-UUU_p2	matK-trnK-UUU	2	CCACAATCATGAGCAAGTGC	ACGGTAGAGTACTCGGCTTT	20	20	50.0	50.0	57.7	58.2	0.1	17.1	0.441789	155779	156548	853-900	1.000000	88.5
matK-trnK-UUU_p3	matK-trnK-UUU	3	CCACAATCATGAGCAAGTGC	GGTGGAACTTCGGCCTAATC	20	20	50.0	55.0	57.7	58.3	0.1	0.0	0.608496	155779	156548	1313-1619	1.000000	87.1
matK-trnK-UUU_p4	matK-trnK-UUU	4	CCACAATCATGAGCAAGTGC	GTGGAACTTCGGCCTAATCA	20	20	50.0	50.0	57.7	57.6	0.1	0.0	0.669959	155779	156548	1312-1618	1.000000	86.6
matK-trnK-UUU_p5	matK-trnK-UUU	5	CCACAATCATGAGCAAGTGC	TGAGGGTTGCTAACTCAACG	20	20	50.0	50.0	57.7	57.8	0.1	2.2	0.437187	155779	156548	917-917	0.500000	56.5
trnK-UUU_p1	trnK-UUU	1	GAAGAACCAAAGCTCGTTGC	GGTGGAACTTCGGCCTAATC	20	20	50.0	55.0	58.0	58.3	0.0	0.0	0.373067	156549	156585	722-1075	1.000000	88.0
trnK-UUU_p2	trnK-UUU	2	AGCCGAATCGCTCTTTTGAT	GGTGGAACTTCGGCCTAATC	20	20	45.0	55.0	58.0	58.3	0.0	0.0	0.364965	156549	156585	921-1275	1.000000	87.6
trnK-UUU_p3	trnK-UUU	3	TCTCCTTCGAATCCCCCTTT	GGTGGAACTTCGGCCTAATC	20	20	50.0	55.0	58.0	58.3	0.0	0.0	0.368281	156549	156585	1023-1023	0.500000	57.1
trnK-UUU_p4	trnK-UUU	4	AGTCGCGGTCTTACAAACTC	GGTGGAACTTCGGCCTAATC	20	20	50.0	55.0	57.9	58.3	6.1	0.0	0.396866	156549	156585	517-517	0.500000	56.8
trnK-UUU_p5	trnK-UUU	5	GTCGCGGTCTTACAAACTCT	GGTGGAACTTCGGCCTAATC	20	20	50.0	55.0	57.9	58.3	6.1	0.0	0.396866	156549	156585	516-516	0.500000	56.8
trnK-UUU-rps16_p1	trnK-UUU-rps16	1	GAAGAACCAAAGCTCGTTGC	GGTGGAACTTCGGCCTAATC	20	20	50.0	55.0	58.0	58.3	0.0	0.0	0.373067	156586	156947	722-1075	1.000000	88.0
trnK-UUU-rps16_p2	trnK-UUU-rps16	2	AGCCGAATCGCTCTTTTGAT	GGTGGAACTTCGGCCTAATC	20	20	45.0	55.0	58.0	58.3	0.0	0.0	0.364965	156586	156947	921-1275	1.000000	87.6
trnK-UUU-rps16_p3	trnK-UUU-rps16	3	TCTCCTTCGAATCCCCCTTT	GGTGGAACTTCGGCCTAATC	20	20	50.0	55.0	58.0	58.3	0.0	0.0	0.368281	156586	156947	1023-1023	0.500000	57.1
trnK-UUU-rps16_p4	trnK-UUU-rps16	4	AGTCGCGGTCTTACAAACTC	GGTGGAACTTCGGCCTAATC	20	20	50.0	55.0	57.9	58.3	6.1	0.0	0.396866	156586	156947	517-517	0.500000	56.8
trnK-UUU-rps16_p5	trnK-UUU-rps16	5	GTCGCGGTCTTACAAACTCT	GGTGGAACTTCGGCCTAATC	20	20	50.0	55.0	57.9	58.3	6.1	0.0	0.396866	156586	156947	516-516	0.500000	56.8