Hotspot

OF286_pgp052

LSC · 30184-30849 · 666 bp

Back to Eutreptiella

Pi 0.323

MarkerSeek score 81.60

Primer available yes

Label OF286_pgp052

Feature Metadata

label_name: OF286_pgp052
feature_type: gene
region: LSC
length_bp: 666
variable_sites: 209
pi: 0.323
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 81.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
OF286_pgp052_p1	CGTGGTCATGTTAAGAGCGA	TCCCCATCACTTCCTGGAAT	50.0	50.0	58.0	58.0	1594 bp	0.5	59.8
OF286_pgp052_p2	TCGTGGTCATGTTAAGAGCG	TCCCCATCACTTCCTGGAAT	50.0	50.0	58.0	58.0	1595 bp	0.5	59.8
OF286_pgp052_p3	GGTCGTGGATTTCCGATTCA	TCCCCATCACTTCCTGGAAT	50.0	50.0	58.0	58.0	1212 bp	0.5	59.7
OF286_pgp052_p4	CGTGGTCATGTTAAGAGCGA	GCTACCGCCAAAGTCCATAA	50.0	50.0	58.0	58.0	1768 bp	0.5	59.7
OF286_pgp052_p5	TCGTGGTCATGTTAAGAGCG	GCTACCGCCAAAGTCCATAA	50.0	50.0	58.0	58.0	1769 bp	0.5	59.7