Hotspot

OF286_pgp032

LSC · 55195-57044 · 1850 bp

Back to Eutreptiella

Pi 0.472

MarkerSeek score 75.00

Primer available yes

Label OF286_pgp032

Feature Metadata

label_name: OF286_pgp032
feature_type: gene
region: LSC
length_bp: 1850
variable_sites: 587
pi: 0.472
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.67
markerseek_score: 75.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
OF286_pgp032_p1	GCGGGAAAAAGACGATCTCT	TGTTCGTGGCTCGTTAGATG	50.0	50.0	58.0	58.0	2889 bp	0.5	59.8
OF286_pgp032_p2	GCGGGAAAAAGACGATCTCT	CGTTAGATGCTGCAGGAGTT	50.0	50.0	58.0	58.0	2878 bp	0.5	59.8
OF286_pgp032_p3	GCGGGAAAAAGACGATCTCT	CGAACAAAGCTTACGCACAG	50.0	50.0	58.0	58.1	2250 bp	0.5	59.4
OF286_pgp032_p4	TTCAACACGAACAGCCTCAA	CGAACAAAGCTTACGCACAG	45.0	50.0	58.0	58.1	2459 bp	0.5	59.2
OF286_pgp032_p5	TTTCAACACGAACAGCCTCA	CGAACAAAGCTTACGCACAG	45.0	50.0	58.0	58.1	2460 bp	0.5	59.2