Hotspot

OF286_pgp030

LSC · 57708-58082 · 375 bp

Back to Eutreptiella

Pi 0.509

MarkerSeek score 72.40

Primer available yes

Label OF286_pgp030

Feature Metadata

label_name: OF286_pgp030
feature_type: gene
region: LSC
length_bp: 375
variable_sites: 137
pi: 0.509
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.72
markerseek_score: 72.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
OF286_pgp030_p1	GGGTCTGGTGTGATTAGACG	TACTCTGTACCGTCCTGCAA	55.0	50.0	57.7	58.1	840 bp	0.5	56.9
OF286_pgp030_p2	GGGTCTGGTGTGATTAGACG	CTGTACCGTCCTGCAAATCA	55.0	50.0	57.7	57.9	836 bp	0.5	56.9
OF286_pgp030_p3	GGGTCTGGTGTGATTAGACG	AAAGGATTTGTAGGCGCACA	55.0	45.0	57.7	58.1	863 bp	0.5	56.9
OF286_pgp030_p4	GGGTCTGGTGTGATTAGACG	TCCGATCTATCCGTCAGGTC	55.0	55.0	57.7	58.1	1085 bp	0.5	56.8
OF286_pgp030_p5	GGGTCTGGTGTGATTAGACG	TCGAATCAATGCTGCCTCAA	55.0	45.0	57.7	57.9	738 bp	0.5	56.7