Hotspot

OF286_pgp025

LSC · 62245-63588 · 1344 bp

Back to Eutreptiella

Pi 0.473

MarkerSeek score 81.00

Primer available yes

Label OF286_pgp025

Feature Metadata

label_name: OF286_pgp025
feature_type: gene
region: LSC
length_bp: 1344
variable_sites: 502
pi: 0.473
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.79
markerseek_score: 81.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
OF286_pgp025_p1	AGTTAACTTTTAGATCCCAATAGG	AATCAATTTTGGAGCAGATTT	33.3	28.6	54.1	52.2	1401 bp	0.5	20.0
OF286_pgp025_p2	GTTAACTTTTAGATCCCAATAGG	AATCAATTTTGGAGCAGATTT	34.8	28.6	52.6	52.2	1400 bp	0.5	20.0
OF286_pgp025_p3	AGTTAACTTTTAGATCCCAATAGG	AATCAATTTTGGAGCAGATTTA	33.3	27.3	54.1	52.3	1401 bp	0.5	20.0
OF286_pgp025_p4	TAGTTAACTTTTAGATCCCAATAGG	AATCAATTTTGGAGCAGATTT	32.0	28.6	54.1	52.2	1402 bp	0.5	20.0
OF286_pgp025_p5	AGTTAACTTTTAGATCCCAATAGG	ATCAATTTTGGAGCAGATTTAT	33.3	27.3	54.1	52.1	1400 bp	0.5	20.0