Hotspot

OF286_pgp009

LSC · 77035-78546 · 1512 bp

Back to Eutreptiella

Pi 0.481

MarkerSeek score 74.40

Primer available yes

Label OF286_pgp009

Feature Metadata

label_name: OF286_pgp009
feature_type: gene
region: LSC
length_bp: 1512
variable_sites: 449
pi: 0.481
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.62
markerseek_score: 74.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
OF286_pgp009_p1	TGGACGAGTATGACCGAAGT	ACATTTGCCTCTGTTGCAGA	50.0	45.0	58.2	58.0	2919 bp	0.5	58.5
OF286_pgp009_p2	TGGACGAGTATGACCGAAGT	GCCTCTGTTGCAGAATCAGA	50.0	50.0	58.2	57.9	2913 bp	0.5	57.8
OF286_pgp009_p3	TGGACGAGTATGACCGAAGT	CAAGGTCTTGTTACCGCAGA	50.0	50.0	58.2	57.8	2655 bp	0.5	57.3
OF286_pgp009_p4	TGGACGAGTATGACCGAAGT	CTCAAGGTCTTGTTACCGCA	50.0	50.0	58.2	57.8	2657 bp	0.5	57.3
OF286_pgp009_p5	TGGACGAGTATGACCGAAGT	TCAAGGTCTTGTTACCGCAG	50.0	50.0	58.2	57.8	2656 bp	0.5	57.3