Hotspot

OF286_pgp004

LSC · 85251-88712 · 3462 bp

Back to Eutreptiella

Pi 0.452

MarkerSeek score 69.10

Primer available yes

Label OF286_pgp004

Feature Metadata

label_name: OF286_pgp004
feature_type: gene
region: LSC
length_bp: 3462
variable_sites: 827
pi: 0.452
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.53
markerseek_score: 69.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
OF286_pgp004_p1	AGCTCCATCCGATAGAACGA	TCTTACCCGGAGAGTTTGGT	50.0	50.0	58.0	58.0	1172 bp	0.5	59.7
OF286_pgp004_p2	AGCTCCATCCGATAGAACGA	CCCGGAGAGTTTGGTTCAAA	50.0	50.0	58.0	58.0	1167 bp	0.5	59.7
OF286_pgp004_p3	AGTGGTTTCGTTCAGCAAGT	TCTTACCCGGAGAGTTTGGT	45.0	50.0	58.0	58.0	1231 bp	0.5	59.5
OF286_pgp004_p4	AGTGGTTTCGTTCAGCAAGT	CCCGGAGAGTTTGGTTCAAA	45.0	50.0	58.0	58.0	1226 bp	0.5	59.5
OF286_pgp004_p5	CGAGACTGTGGTTCAACGAT	TCTTACCCGGAGAGTTTGGT	50.0	50.0	57.9	58.0	1264 bp	0.5	59.3