Hotspot

trnC-OF286_pgp004

LSC · 84129-85250 · 1122 bp

Back to Eutreptiella

Pi 0.466

MarkerSeek score 74.00

Primer available yes

Label trnC-OF286_pgp004

Feature Metadata

label_name: trnC-OF286_pgp004
feature_type: igs
region: LSC
length_bp: 1122
variable_sites: 315
pi: 0.466
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.60
markerseek_score: 74.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-OF286_pgp004_p1	CGGTGCCATTTTTGGATTCC	GAATAAGGGCCTCGAACGTT	50.0	50.0	58.0	58.0	2168 bp	0.5	59.8
trnC-OF286_pgp004_p2	TTCCAGCGACGTACTTGATG	GAATAAGGGCCTCGAACGTT	50.0	50.0	58.0	58.0	2152 bp	0.5	59.8
trnC-OF286_pgp004_p3	CGGTGCCATTTTTGGATTCC	AGCGGCACTATAAGTTTGGG	50.0	50.0	58.0	58.0	1741 bp	0.5	59.6
trnC-OF286_pgp004_p4	CGGTGCCATTTTTGGATTCC	CGGCACTATAAGTTTGGGCT	50.0	50.0	58.0	58.0	1739 bp	0.5	59.6
trnC-OF286_pgp004_p5	TTCCAGCGACGTACTTGATG	CGGCACTATAAGTTTGGGCT	50.0	50.0	58.0	58.0	1723 bp	0.5	59.6