Hotspot

OF286_pgp025-OF286_pgp024

LSC · 63589-64275 · 687 bp

Back to Eutreptiella

Pi 0.215

MarkerSeek score 84.60

Primer available yes

Label OF286_pgp025-OF286_pgp024

Feature Metadata

label_name: OF286_pgp025-OF286_pgp024
feature_type: igs
region: LSC
length_bp: 687
variable_sites: 141
pi: 0.215
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 84.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
OF286_pgp025-OF286_pgp024_p1	ACCGGCGTTTAACAGTTTCT	TTGCAAGTTGACCGAAGTGA	45.0	45.0	58.0	58.0	1263 bp	0.5	59.4
OF286_pgp025-OF286_pgp024_p2	ACCGGCGTTTAACAGTTTCT	ATTAAGTGACCCGTCCATGC	45.0	50.0	58.0	58.0	1681 bp	0.5	59.4
OF286_pgp025-OF286_pgp024_p3	ACCGGCGTTTAACAGTTTCT	GGGTGCGGTGATGTACTAAG	45.0	55.0	58.0	58.1	1753 bp	0.5	59.1
OF286_pgp025-OF286_pgp024_p4	ACCGGCGTTTAACAGTTTCT	GGTGCGGTGATGTACTAAGG	45.0	55.0	58.0	58.1	1752 bp	0.5	59.1
OF286_pgp025-OF286_pgp024_p5	ATGTCCAGACGAAAAGAGCC	TTGCAAGTTGACCGAAGTGA	50.0	45.0	57.9	58.0	1241 bp	0.5	58.9