Hotspot

trnfM-trnS

LSC · 54113-54220 · 108 bp

Back to Eutreptiella

Pi 0.47

MarkerSeek score 72.20

Primer available yes

Label trnfM-trnS

Feature Metadata

label_name: trnfM-trnS
feature_type: igs
region: LSC
length_bp: 108
variable_sites: 39
pi: 0.47
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.77
markerseek_score: 72.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnfM-trnS_p1	TGATGTCGTGGGTTCGAATC	AGAGATCGTCTTTTTCCCGC	50.0	50.0	58.0	58.0	936 bp	0.5	59.9
trnfM-trnS_p2	AGGGGCTAGAGCATCGTATT	AGAGATCGTCTTTTTCCCGC	50.0	50.0	58.0	58.0	1042 bp	0.5	59.8
trnfM-trnS_p3	TGATGTCGTGGGTTCGAATC	TGAGGCTGTTCGTGTTGAAA	50.0	45.0	58.0	58.0	726 bp	0.5	59.7
trnfM-trnS_p4	TGATGTCGTGGGTTCGAATC	TTGAGGCTGTTCGTGTTGAA	50.0	45.0	58.0	58.0	727 bp	0.5	59.7
trnfM-trnS_p5	TGATGTCGTGGGTTCGAATC	GTACCAAGGGTTCGAATCCC	50.0	55.0	58.0	58.0	263 bp	0.5	59.7