Hotspot

OF286_pgp037-trnL

LSC · 48932-50939 · 2008 bp

Back to Eutreptiella

Pi 0.229

MarkerSeek score 80.90

Primer available yes

Label OF286_pgp037-trnL

Feature Metadata

label_name: OF286_pgp037-trnL
feature_type: igs
region: LSC
length_bp: 2008
variable_sites: 410
pi: 0.229
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.89
markerseek_score: 80.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
OF286_pgp037-trnL_p1	TGGGATTGTTACTCGAGGGT	CTACCATTCCACCACGAAGG	50.0	55.0	58.1	58.0	2051 bp	0.5	59.4
OF286_pgp037-trnL_p2	CACCCTAGTGTCCGTTATGC	CTACCATTCCACCACGAAGG	55.0	55.0	58.1	58.0	2680 bp	0.5	59.3
OF286_pgp037-trnL_p3	GTTCTCAGAGCCACATTCGT	CTACCATTCCACCACGAAGG	50.0	55.0	57.9	58.0	2762 bp	0.5	59.1
OF286_pgp037-trnL_p4	TGGGATTGTTACTCGAGGGT	TAGACGTTAACAAGCCACGG	50.0	50.0	58.1	57.9	2307 bp	0.5	59.0
OF286_pgp037-trnL_p5	CCTCGCCATAATAGCTGCAT	CTACCATTCCACCACGAAGG	50.0	55.0	57.9	58.0	2077 bp	0.5	59.0