Hotspot

OF286_pgp043-OF286_pgp042

LSC · 39145-39754 · 610 bp

Back to Eutreptiella

Pi 0.466

MarkerSeek score 83.10

Primer available yes

Label OF286_pgp043-OF286_pgp042

Feature Metadata

label_name: OF286_pgp043-OF286_pgp042
feature_type: igs
region: LSC
length_bp: 610
variable_sites: 265
pi: 0.466
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 83.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
OF286_pgp043-OF286_pgp042_p1	TCTGACGGTATGCCTCTAGG	CCGAGTGTGTGGGACTATTG	55.0	55.0	58.0	58.0	1886 bp	0.5	59.8
OF286_pgp043-OF286_pgp042_p2	AACGCTCACAACTTCCCATT	CCGAGTGTGTGGGACTATTG	45.0	55.0	58.0	58.0	1388 bp	0.5	59.8
OF286_pgp043-OF286_pgp042_p3	TCTGACGGTATGCCTCTAGG	ATTCGAAGGACGTGACGAAG	55.0	50.0	58.0	58.0	1325 bp	0.5	59.7
OF286_pgp043-OF286_pgp042_p4	AACGCTCACAACTTCCCATT	ATTCGAAGGACGTGACGAAG	45.0	50.0	58.0	58.0	827 bp	0.5	59.7
OF286_pgp043-OF286_pgp042_p5	GGTGTTTTCGGTGGTTCTCT	CCGAGTGTGTGGGACTATTG	50.0	55.0	58.0	58.0	1781 bp	0.5	59.6