Hotspot

ycf1

SSC · 123072-128384 · 5313 bp

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Pi 0.0211

MarkerSeek score 56.00

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5313
variable_sites: 272
pi: 0.0211
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	AATTTGTATGACCATCGAGG	TCAGAACCCCAAGAAGAAAA	40.0	40.0	53.1	54.4	161 bp	1.0	48.0
ycf1_p2	AATTTGTATGACCATCGAGG	TTCAGAACCCCAAGAAGAAA	40.0	40.0	53.1	54.4	162 bp	1.0	48.0
ycf1_p3	AATTTGTATGACCATCGAGG	TTTCAGAACCCCAAGAAGAA	40.0	40.0	53.1	54.4	163 bp	1.0	48.0
ycf1_p4	AATTTGTATGACCATCGAGG	TTTTCAGAACCCCAAGAAGA	40.0	40.0	53.1	54.4	164 bp	1.0	48.0
ycf1_p5	AATTTGTATGACCATCGAGG	TCAGAACCCCAAGAAGAAAAA	40.0	38.1	53.1	55.2	161 bp	1.0	48.0