Hotspot

rpl32-trnL-TAG

SSC · 112797-113533 · 737 bp

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Pi 0.0442
MarkerSeek score 80.60
Primer available yes
Label rpl32-trnL-TAG

Feature Metadata

label_name
rpl32-trnL-TAG
feature_type
igs
region
SSC
length_bp
737
variable_sites
82
pi
0.0442
unique_haplotype_count
8
primer_available
yes
alignment_reliability
0.94
markerseek_score
80.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-TAG_p1 GGAAGGGATATTGGACATCGT TCCTAAGAGCAGCGTGTCTA 47.6 50.0 57.2 57.9 783-873 bp 1.0 76.8
rpl32-trnL-TAG_p2 GGAAGGGATATTGGACATCGT TAACCACTGGGACTGGAGAG 47.6 55.0 57.2 58.1 1360-1447 bp 1.0 75.2
rpl32-trnL-TAG_p3 GGAAGGGATATTGGACATCGT TATTGCAGGCTGAGGCATTT 47.6 45.0 57.2 57.9 1330-1417 bp 1.0 74.6
rpl32-trnL-TAG_p4 GGAAGGGATATTGGACATCGT CTCGCACTGCTTCCTAAGAG 47.6 55.0 57.2 58.1 794-884 bp 0.75 66.5
rpl32-trnL-TAG_p5 GGAAGGGATATTGGACATCGT CTCTCGCACTGCTTCCTAAG 47.6 55.0 57.2 58.1 796-886 bp 0.75 66.5