Hotspot

rpl36-rps8

LSC · 79230-79695 · 466 bp

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Pi 0.0437

MarkerSeek score 82.30

Primer available yes

Label rpl36-rps8

Feature Metadata

label_name: rpl36-rps8
feature_type: igs
region: LSC
length_bp: 466
variable_sites: 47
pi: 0.0437
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 82.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl36-rps8_p1	TTTTACGAACGGAAGCCCTT	TCGGCGGAGAAATTTTGTGT	45.0	45.0	57.7	58.4	500-539 bp	1.0	85.9
rpl36-rps8_p2	TTTTACGAACGGAAGCCCTT	CGGCGGAGAAATTTTGTGTT	45.0	45.0	57.7	57.6	499-538 bp	1.0	85.8
rpl36-rps8_p3	ACGAACGGAAGCCCTTATTT	TCGGCGGAGAAATTTTGTGT	45.0	45.0	57.5	58.4	496-535 bp	1.0	84.1
rpl36-rps8_p4	ATTTTACGAACGGAAGCCCT	TCGGCGGAGAAATTTTGTGT	45.0	45.0	57.5	58.4	501-540 bp	1.0	84.0
rpl36-rps8_p5	ACGAACGGAAGCCCTTATTT	CGGCGGAGAAATTTTGTGTT	45.0	45.0	57.5	57.6	495-534 bp	1.0	84.0