Hotspot

rpl20-rps12

LSC · 68396-69179 · 784 bp

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Pi 0.018

MarkerSeek score 67.60

Primer available yes

Label rpl20-rps12

Feature Metadata

label_name: rpl20-rps12
feature_type: igs
region: LSC
length_bp: 784
variable_sites: 37
pi: 0.018
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 67.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl20-rps12_p1	TCCGAGCTATATATCCGCGT	GAGGAACATGTACTCGGGTG	50.0	55.0	58.0	57.7	818-841 bp	1.0	84.8
rpl20-rps12_p2	TTTTGTTCTACGCCTCCGAG	GAGGAACATGTACTCGGGTG	50.0	55.0	57.9	57.7	832-855 bp	1.0	84.4
rpl20-rps12_p3	TCCGAGCTATATATCCGCGT	AGGAACATGTACTCGGGTGT	50.0	50.0	58.0	58.4	817-840 bp	1.0	84.3
rpl20-rps12_p4	TTTTGTTCTACGCCTCCGAG	AGGAACATGTACTCGGGTGT	50.0	50.0	57.9	58.4	831-854 bp	1.0	83.9
rpl20-rps12_p5	TCCGAGCTATATATCCGCGT	GCGACGAGGAACATGTACTC	50.0	55.0	58.0	58.5	823-846 bp	1.0	83.5