Hotspot

psaJ-rpl33

LSC · 66543-66977 · 435 bp

Back to Eutrema

Pi 0.0457

MarkerSeek score 72.50

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 435
variable_sites: 41
pi: 0.0457
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 72.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	CGAACTGAACTAAGAGCGCT	GGGGTGTTATGCCGATTCTT	50.0	50.0	58.0	57.9	1177-1321 bp	1.0	88.3
psaJ-rpl33_p2	CGAACTGAACTAAGAGCGCT	TCCAATCGACTAGGGGTGTT	50.0	50.0	58.0	58.1	1189-1333 bp	1.0	88.3
psaJ-rpl33_p3	CGAACTGAACTAAGAGCGCT	TTCCAATCGACTAGGGGTGT	50.0	50.0	58.0	58.1	1190-1334 bp	1.0	88.3
psaJ-rpl33_p4	GTTTTGGAGACCCACGTTCT	GGGGTGTTATGCCGATTCTT	50.0	50.0	58.0	57.9	1199-1343 bp	1.0	88.1
psaJ-rpl33_p5	GTTTTGGAGACCCACGTTCT	TCCAATCGACTAGGGGTGTT	50.0	50.0	58.0	58.1	1211-1355 bp	1.0	88.0