Hotspot

petG-trnW-CCA

LSC · 65745-65873 · 129 bp

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Pi 0.0543

MarkerSeek score 66.80

Primer available yes

Label petG-trnW-CCA

Feature Metadata

label_name: petG-trnW-CCA
feature_type: igs
region: LSC
length_bp: 129
variable_sites: 16
pi: 0.0543
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petG-trnW-CCA_p1	CGTGGTGATCAGTTGGACTT	AGAACGTGGGTCTCCAAAAC	50.0	50.0	57.8	58.0	206 bp	1.0	84.6
petG-trnW-CCA_p2	ACAGACGTGGTGATCAGTTG	AGAACGTGGGTCTCCAAAAC	50.0	50.0	57.8	58.0	211 bp	1.0	84.5
petG-trnW-CCA_p3	ACAGACGTGGTGATCAGTTG	AGCGCTCTTAGTTCAGTTCG	50.0	50.0	57.8	58.0	233 bp	1.0	84.1
petG-trnW-CCA_p4	CGTGGTGATCAGTTGGACTT	AGCGCTCTTAGTTCAGTTCG	50.0	50.0	57.8	58.0	228 bp	1.0	84.1
petG-trnW-CCA_p5	ACAGACGTGGTGATCAGTTG	CGCTCTTAGTTCAGTTCGGT	50.0	50.0	57.8	57.7	231 bp	1.0	81.5