Hotspot

petA-psbJ

LSC · 62262-63204 · 943 bp

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Pi 0.0291

MarkerSeek score 66.40

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 943
variable_sites: 67
pi: 0.0291
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	TGAGAAGGTTCAATTATCCGA	GGATTAGGTTCATCCCTGTAG	38.1	47.6	54.4	54.9	998-1083 bp	1.0	47.5
petA-psbJ_p2	AGAAACAGTTTGAGAAGGTTCA	GGATTAGGTTCATCCCTGTAG	36.4	47.6	55.8	54.9	1008-1093 bp	1.0	47.4
petA-psbJ_p3	AAGAAACAGTTTGAGAAGGTTCA	GGATTAGGTTCATCCCTGTAG	34.8	47.6	56.5	54.9	1009-1094 bp	1.0	47.4
petA-psbJ_p4	AGAAACAGTTTGAGAAGGTTCAA	GGATTAGGTTCATCCCTGTAG	34.8	47.6	56.5	54.9	1008-1093 bp	1.0	47.4
petA-psbJ_p5	AGAAACAGTTTGAGAAGGTTCA	GGATTAGGTTCATCCCTGTA	36.4	45.0	55.8	53.5	1008-1093 bp	1.0	47.4