Hotspot

trnF-GAA-ndhJ

LSC · 47800-48280 · 481 bp

Back to Eutrema

Pi 0.0322

MarkerSeek score 71.30

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 481
variable_sites: 40
pi: 0.0322
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 71.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	CCAAGAAATTCCCGGTCCAA	CATCCACGACTGAAACGGAT	50.0	50.0	58.1	58.0	834-1296 bp	1.0	88.1
trnF-GAA-ndhJ_p2	AGGGATAGGTGCAGAGACTC	CATCCACGACTGAAACGGAT	55.0	50.0	57.9	58.0	1303-1678 bp	1.0	87.5
trnF-GAA-ndhJ_p3	GGCCAGGATAACTCAGTTGG	CATCCACGACTGAAACGGAT	55.0	50.0	58.0	58.0	728 bp	0.375	54.9
trnF-GAA-ndhJ_p4	GGCCAGGATAACTCAGTTGG	CCCGAAAGTTGGATAGGGTG	55.0	55.0	58.0	58.0	701 bp	0.375	54.7
trnF-GAA-ndhJ_p5	GGCCAGGATAACTCAGTTGG	CCGAAAGTTGGATAGGGTGG	55.0	55.0	58.0	58.0	700 bp	0.375	54.7