Hotspot

trnE-TTC-trnT-GGT

LSC · 30564-31380 · 817 bp

Back to Eutrema

Pi 0.0324

MarkerSeek score 79.40

Primer available yes

Label trnE-TTC-trnT-GGT

Feature Metadata

label_name: trnE-TTC-trnT-GGT
feature_type: igs
region: LSC
length_bp: 817
variable_sites: 61
pi: 0.0324
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 79.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-TTC-trnT-GGT_p1	TTTCGTAGTACCCTACCCCC	GAACCGATGACTTACGCCTT	55.0	50.0	57.8	58.0	691-977 bp	1.0	87.9
trnE-TTC-trnT-GGT_p2	TTTCGTAGTACCCTACCCCC	CCATGGCGTTACTCTACCAC	55.0	55.0	57.8	58.1	670-956 bp	1.0	87.6
trnE-TTC-trnT-GGT_p3	TTTCGTAGTACCCTACCCCC	TGGCGTTACTCTACCACTGA	55.0	50.0	57.8	58.1	667-953 bp	1.0	87.4
trnE-TTC-trnT-GGT_p4	GCTGCCTCCTTGAAAGAGAG	GAACCGATGACTTACGCCTT	55.0	50.0	58.3	58.0	653-939 bp	1.0	87.3
trnE-TTC-trnT-GGT_p5	TGTCCTGAACCACTAGACGA	GAACCGATGACTTACGCCTT	50.0	50.0	57.7	58.0	632-918 bp	1.0	87.3