Hotspot

psbM-trnD-GTC

LSC · 28811-29841 · 1031 bp

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Pi 0.0273

MarkerSeek score 71.10

Primer available yes

Label psbM-trnD-GTC

Feature Metadata

label_name: psbM-trnD-GTC
feature_type: igs
region: LSC
length_bp: 1031
variable_sites: 64
pi: 0.0273
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 71.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GTC_p1	TGAGAATGAAGAGTGCAGTAGC	GTTCGAGCCCCGTCAGTC	45.5	66.7	58.1	60.1	1085-1103 bp	1.0	46.9
psbM-trnD-GTC_p2	GAGAATGAAGAGTGCAGTAGC	GTTCGAGCCCCGTCAGTC	47.6	66.7	56.4	60.1	1084-1102 bp	1.0	46.9
psbM-trnD-GTC_p3	AGGAATGAGAATGAAGAGTGCA	GTTCGAGCCCCGTCAGTC	40.9	66.7	57.4	60.1	1090-1108 bp	1.0	46.8
psbM-trnD-GTC_p4	TGAGAATGAAGAGTGCAGTAGC	GAGCACCGCCCTGTCAAG	45.5	66.7	58.1	60.7	1116-1134 bp	1.0	46.7
psbM-trnD-GTC_p5	TGAGAATGAAGAGTGCAGTAGC	GCCCTGTCAAGGCGGAAG	45.5	66.7	58.1	60.7	1109-1127 bp	1.0	46.7