Hotspot

rpoB-trnC-GCA

LSC · 26263-27374 · 1112 bp

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Pi 0.0314

MarkerSeek score 74.00

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1112
variable_sites: 88
pi: 0.0314
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 74.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	CACGAGCCCATATTCTTGCT	ATGTCTGTGCATTCCGAGAC	50.0	50.0	58.0	58.0	1969-2001 bp	0.75	74.6
rpoB-trnC-GCA_p2	ATTGGATCCCCACCTACACA	ATGTCTGTGCATTCCGAGAC	50.0	50.0	58.0	58.0	2168-2200 bp	0.75	74.4
rpoB-trnC-GCA_p3	ATTGGATCCCCACCTACACA	CTCCAAGGAATCTGCCTCAC	50.0	55.0	58.0	58.0	2283 bp	0.375	54.8
rpoB-trnC-GCA_p4	ATTGGATCCCCACCTACACA	AGTCTCCAAGGAATCTGCCT	50.0	50.0	58.0	58.0	2286 bp	0.375	54.8
rpoB-trnC-GCA_p5	ATTGGATCCCCACCTACACA	TCTCCAAGGAATCTGCCTCA	50.0	50.0	58.0	58.0	2284 bp	0.375	54.7