Hotspot

trnR-TCT-atpA

LSC · 9549-9853 · 305 bp

Back to Eutrema

Pi 0.0468

MarkerSeek score 79.10

Primer available yes

Label trnR-TCT-atpA

Feature Metadata

label_name: trnR-TCT-atpA
feature_type: igs
region: LSC
length_bp: 305
variable_sites: 32
pi: 0.0468
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 79.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-TCT-atpA_p1	GCGAAAAGCGTCCATTGTC	TCAAGAGCAACTGGAACGTT	52.6	45.0	58.0	57.7	422-454 bp	1.0	77.2
trnR-TCT-atpA_p2	GCGAAAAGCGTCCATTGTC	TTCAAGAGCAACTGGAACGT	52.6	45.0	58.0	57.7	423-455 bp	1.0	77.2
trnR-TCT-atpA_p3	GCGAAAAGCGTCCATTGTCT	TCAAGAGCAACTGGAACGTT	50.0	45.0	59.5	57.7	422-454 bp	1.0	73.3
trnR-TCT-atpA_p4	GCGAAAAGCGTCCATTGTCT	TTCAAGAGCAACTGGAACGT	50.0	45.0	59.5	57.7	423-455 bp	1.0	73.3
trnR-TCT-atpA_p5	GGTTCAAATCCTATTGGACGC	TCAAGAGCAACTGGAACGTT	47.6	45.0	57.3	57.7	365-397 bp	1.0	72.8