Hotspot

trnS-GCT-trnR-TCT

LSC · 7971-9476 · 1506 bp

Back to Eutrema

Pi 0.0265

MarkerSeek score 66.10

Primer available yes

Label trnS-GCT-trnR-TCT

Feature Metadata

label_name: trnS-GCT-trnR-TCT
feature_type: igs
region: LSC
length_bp: 1506
variable_sites: 99
pi: 0.0265
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 66.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCT-trnR-TCT_p1	GCTTTAGTCCACTCAGCCAT	TGATATTGCGTCCAATAGGA	50.0	40.0	57.6	53.9	1610-1765 bp	1.0	52.8
trnS-GCT-trnR-TCT_p2	AGTCCACTCAGCCATCTCTC	TGATATTGCGTCCAATAGGA	55.0	40.0	58.5	53.9	1605-1760 bp	1.0	51.9
trnS-GCT-trnR-TCT_p3	GCTTTAGTCCACTCAGCCAT	TTGATATTGCGTCCAATAGGA	50.0	38.1	57.6	54.8	1611-1766 bp	1.0	51.8
trnS-GCT-trnR-TCT_p4	AGTCCACTCAGCCATCTCTC	TTGATATTGCGTCCAATAGGA	55.0	38.1	58.5	54.8	1606-1761 bp	1.0	50.9
trnS-GCT-trnR-TCT_p5	GCTTTAGTCCACTCAGCCAT	AGGTTTAGAAGACCTATGTCCT	50.0	40.9	57.6	55.6	1574-1729 bp	1.0	50.5