Hotspot

trnH-GTG-psbA

LSC · 77-376 · 300 bp

Back to Eutrema

Pi 0.0595

MarkerSeek score 78.40

Primer available yes

Label trnH-GTG-psbA

Feature Metadata

label_name: trnH-GTG-psbA
feature_type: igs
region: LSC
length_bp: 300
variable_sites: 44
pi: 0.0595
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 78.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GTG-psbA_p1	CTGCCTTAATCCACTTGGCT	CCTCTAGACCTAGCTGCTGT	50.0	55.0	57.9	57.9	329-374 bp	1.0	90.4
trnH-GTG-psbA_p2	CTGCCTTAATCCACTTGGCT	TTCCCTCTAGACCTAGCTGC	50.0	55.0	57.9	57.9	332-377 bp	1.0	90.3
trnH-GTG-psbA_p3	ACTGCCTTAATCCACTTGGC	CCTCTAGACCTAGCTGCTGT	50.0	55.0	58.2	57.9	330-375 bp	1.0	90.2
trnH-GTG-psbA_p4	ACTGCCTTAATCCACTTGGC	TTCCCTCTAGACCTAGCTGC	50.0	55.0	58.2	57.9	333-378 bp	1.0	90.1
trnH-GTG-psbA_p5	AATCCACTTGGCTACATCCG	CCTCTAGACCTAGCTGCTGT	50.0	55.0	57.7	57.9	322-367 bp	1.0	88.8