Hotspot

psbA

LSC · 130-1191 · 1062 bp

Back to Euterpe

Pi 0.00126

MarkerSeek score 36.20

Primer available yes

Label psbA

Feature Metadata

label_name: psbA
feature_type: gene
region: LSC
length_bp: 1062
variable_sites: 2
pi: 0.00126
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 36.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA_p1	TGGGTATTGCTCCATTGTTTGA	GTGCTTGGGAGTCCTTGAAA	40.9	50.0	58.2	58.0	1159 bp	1.0	62.2
psbA_p2	TGGGTATTGCTCCATTGTTTG	GTGCTTGGGAGTCCTTGAAA	42.9	50.0	56.8	58.0	1159 bp	1.0	62.1
psbA_p3	TGGGTATTGCTCCATTGTTTGA	AATACGTGTGCTTGGGAGTC	40.9	50.0	58.2	57.9	1166 bp	1.0	61.6
psbA_p4	TGGGTATTGCTCCATTGTTTG	AATACGTGTGCTTGGGAGTC	42.9	50.0	56.8	57.9	1166 bp	1.0	61.5
psbA_p5	GGGTATTGCTCCATTGTTTGA	GTGCTTGGGAGTCCTTGAAA	42.9	50.0	56.5	58.0	1158 bp	1.0	59.9