Hotspot

rpl32-trnL-UAG

SSC · 116544-117330 · 787 bp

Back to Euterpe

Pi 0.00593
MarkerSeek score 39.00
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
787
variable_sites
7
pi
0.00593
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
39.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 TTTTTGTGCGACAAACAAAT AGCGTGTCTACCGATTTCAC 30.0 50.0 53.4 58.0 854-857 bp 1.0 43.6
rpl32-trnL-UAG_p2 TTTTTGTGCGACAAACAAAT CAGCGTGTCTACCGATTTCA 30.0 50.0 53.4 58.0 855-858 bp 1.0 43.6
rpl32-trnL-UAG_p3 TTTTTGTGCGACAAACAAAT TCCTAAGAGCAGCGTGTCTA 30.0 50.0 53.4 57.9 864-867 bp 1.0 42.7
rpl32-trnL-UAG_p4 TTTTTGTGCGACAAACAAAT CCTAAGAGCAGCGTGTCTAC 30.0 55.0 53.4 57.8 863-866 bp 1.0 42.1
rpl32-trnL-UAG_p5 TTTTTGTGCGACAAACAAAT CTAAGAGCAGCGTGTCTACC 30.0 55.0 53.4 57.8 862-865 bp 1.0 42.1