Hotspot

psaJ-rpl33

LSC · 69534-70086 · 553 bp

Back to Euterpe

Pi 0.00363

MarkerSeek score 60.40

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 553
variable_sites: 3
pi: 0.00363
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	TGGTTTGGGTCTTTAGCAGG	TGACACCATTTCGGACACAA	50.0	45.0	57.7	57.7	705-707 bp	1.0	75.6
psaJ-rpl33_p2	TGGTTTGGGTCTTTAGCAGG	TTGACACCATTTCGGACACA	50.0	45.0	57.7	57.7	706-708 bp	1.0	75.6
psaJ-rpl33_p3	GGTTTGGGTCTTTAGCAGGT	TGACACCATTTCGGACACAA	50.0	45.0	57.7	57.7	704-706 bp	1.0	75.6
psaJ-rpl33_p4	GGTTTGGGTCTTTAGCAGGT	TTGACACCATTTCGGACACA	50.0	45.0	57.7	57.7	705-707 bp	1.0	75.6
psaJ-rpl33_p5	TGGTTTGGGTCTTTAGCAGG	ACACCATTTCGGACACAACT	50.0	45.0	57.7	57.7	703-705 bp	1.0	75.5