Hotspot

petA-psbJ

LSC · 65199-66135 · 937 bp

Back to Euterpe

Pi 0.0164

MarkerSeek score 68.50

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 937
variable_sites: 23
pi: 0.0164
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	ATCCATTACGTGTCCAAGGC	GGTTCATATTCCGGGTTGGG	50.0	55.0	58.0	58.3	1089-1168 bp	1.0	78.9
petA-psbJ_p2	ATCCATTACGTGTCCAAGGC	CATATTCCGGGTTGGGTTCA	50.0	50.0	58.0	57.6	1085-1164 bp	1.0	78.0
petA-psbJ_p3	GGCCTTTTGTTCTTCTTGGC	GGTTCATATTCCGGGTTGGG	50.0	55.0	57.8	58.3	1072-1151 bp	1.0	78.0
petA-psbJ_p4	ATCCATTACGTGTCCAAGGC	GTTCATATTCCGGGTTGGGT	50.0	50.0	58.0	57.6	1088-1167 bp	1.0	77.9
petA-psbJ_p5	CATTACGTGTCCAAGGCCTT	GGTTCATATTCCGGGTTGGG	50.0	55.0	58.2	58.3	1086-1165 bp	1.0	77.8