Hotspot

trnM-CAU-atpE

LSC · 54693-54871 · 179 bp

Back to Euterpe

Pi 0.0708

MarkerSeek score 65.40

Primer available yes

Label trnM-CAU-atpE

Feature Metadata

label_name: trnM-CAU-atpE
feature_type: igs
region: LSC
length_bp: 179
variable_sites: 19
pi: 0.0708
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnM-CAU-atpE_p1	TATTGCTTTCATACGGCGGG	TAGGACACGAGTAGAGGCTG	50.0	55.0	58.4	58.0	268-279 bp	1.0	81.9
trnM-CAU-atpE_p2	TATTGCTTTCATACGGCGGG	CTCTCAGACGAGCTAGGACA	50.0	55.0	58.4	58.0	281-292 bp	1.0	81.7
trnM-CAU-atpE_p3	TATTGCTTTCATACGGCGGG	GCGAATCTAGCTCTCAGACG	50.0	55.0	58.4	58.0	291-302 bp	1.0	81.6
trnM-CAU-atpE_p4	TATTGCTTTCATACGGCGGG	CGAGCTAGGACACGAGTAGA	50.0	55.0	58.4	58.1	273-284 bp	1.0	81.6
trnM-CAU-atpE_p5	TATTGCTTTCATACGGCGGG	GGACACGAGTAGAGGCTGTA	50.0	55.0	58.4	58.3	266-277 bp	1.0	80.3