Hotspot

trnC-GCA-petN

LSC · 28764-29896 · 1133 bp

Back to Euterpe

Pi 0.00177

MarkerSeek score 60.20

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 1133
variable_sites: 3
pi: 0.00177
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	GGGGGACTGCAAATCCTTTA	TAAAGCAGCCCAAGTGAGAC	50.0	50.0	57.5	57.8	1210-1213 bp	1.0	74.7
trnC-GCA-petN_p2	GGGGGACTGCAAATCCTTTA	ACCATTAAAGCAGCCCAAGT	50.0	45.0	57.5	57.7	1215-1218 bp	1.0	73.7
trnC-GCA-petN_p3	GGGGGACTGCAAATCCTTTA	AGAGTCCACTTCTTCCCCAT	50.0	50.0	57.5	57.6	1265-1268 bp	1.0	73.4
trnC-GCA-petN_p4	CCAGTTCAAATCCGGGTGTC	TAAAGCAGCCCAAGTGAGAC	55.0	50.0	58.8	57.8	1187-1190 bp	1.0	72.2
trnC-GCA-petN_p5	GGGGGACTGCAAATCCTTTA	GCAGCCCAAGTGAGACTTAC	50.0	55.0	57.5	58.6	1206-1209 bp	1.0	71.7