Hotspot

rps16-trnQ-UUG

LSC · 5941-7057 · 1117 bp

Back to Euterpe

Pi 0.00418
MarkerSeek score 61.80
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
1117
variable_sites
7
pi
0.00418
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
61.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 CAGCCGATCATGTCCTTCAA GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.0 58.1 1204-1208 bp 1.0 79.9
rps16-trnQ-UUG_p2 GCCGATCATGTCCTTCAAGT GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.0 58.1 1202-1206 bp 1.0 79.9
rps16-trnQ-UUG_p3 AATCCACAGCCGATCATGTC GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.0 58.1 1210-1214 bp 1.0 79.8
rps16-trnQ-UUG_p4 GAATCCACAGCCGATCATGT GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.0 58.1 1211-1215 bp 1.0 79.8
rps16-trnQ-UUG_p5 CAGCCGATCATGTCCTTCAA GAAATTCGAAATGGGGCGTG 50.0 50.0 58.0 58.1 1263-1267 bp 1.0 79.7