Hotspot

rbcL

LSC · 57622-59049 · 1428 bp

Back to Eurya

Pi 0.00112
MarkerSeek score 45.00
Primer available yes
Label rbcL

Feature Metadata

label_name
rbcL
feature_type
gene
region
LSC
length_bp
1428
variable_sites
3
pi
0.00112
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
45.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rbcL_p1 TCGAGTAGACCTTGTTGTTGT TGGTAAAAGATTGGGCCGAG 42.9 50.0 56.9 57.9 1548 bp 1.0 62.5
rbcL_p2 TCGAGTAGACCTTGTTGTTGT GGTAAAAGATTGGGCCGAGT 42.9 50.0 56.9 57.9 1547 bp 1.0 62.4
rbcL_p3 CGAGTAGACCTTGTTGTTGTG TGGTAAAAGATTGGGCCGAG 47.6 50.0 56.9 57.9 1547 bp 1.0 62.1
rbcL_p4 CGAGTAGACCTTGTTGTTGTG GGTAAAAGATTGGGCCGAGT 47.6 50.0 56.9 57.9 1546 bp 1.0 62.1
rbcL_p5 TCGAGTAGACCTTGTTGTTGT TTCGGCTCAATCCTTTTGGT 42.9 45.0 56.9 57.7 1564 bp 1.0 61.1