Hotspot

ndhF-rpl32

SSC · 115367-116280 · 914 bp

Back to Eurya

Pi 0.000438
MarkerSeek score 48.00
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
914
variable_sites
1
pi
0.000438
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
48.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 ACAAAAGGTATGATCCACGCA AGCTTTTAAGGCTGCCCAAT 42.9 45.0 57.9 58.1 1047-1048 bp 1.0 71.2
ndhF-rpl32_p2 ACAAAAGGTATGATCCACGCA AAAGCTTTTAAGGCTGCCCA 42.9 45.0 57.9 58.3 1049-1050 bp 1.0 69.3
ndhF-rpl32_p3 ACAAAAGGTATGATCCACGCA AAGCTTTTAAGGCTGCCCAA 42.9 45.0 57.9 58.3 1048-1049 bp 1.0 69.3
ndhF-rpl32_p4 ACAAAAGGTATGATCCACGCA AGGCTGCCCAATATCCTTTC 42.9 50.0 57.9 57.6 1039-1040 bp 1.0 68.7
ndhF-rpl32_p5 ACAAAAGGTATGATCCACGCA GCTGCCCAATATCCTTTCCT 42.9 50.0 57.9 57.6 1037-1038 bp 1.0 68.7