Hotspot

petA-psbJ

LSC · 65794-66788 · 995 bp

Back to Eurya

Pi 0.00121
MarkerSeek score 50.80
Primer available yes
Label petA-psbJ

Feature Metadata

label_name
petA-psbJ
feature_type
igs
region
LSC
length_bp
995
variable_sites
3
pi
0.00121
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
50.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petA-psbJ_p1 CCATTACGTGTTCAAGGCCT ATTCCGGATTGGGTTCATCC 50.0 50.0 58.2 57.6 1138 bp 1.0 75.9
petA-psbJ_p2 TCCATTACGTGTTCAAGGCC ATTCCGGATTGGGTTCATCC 50.0 50.0 58.2 57.6 1139 bp 1.0 75.8
petA-psbJ_p3 GCATCTGTTGTTTTGGCACA ATTCCGGATTGGGTTCATCC 45.0 50.0 57.8 57.6 1105 bp 1.0 75.5
petA-psbJ_p4 AGCATCTGTTGTTTTGGCAC ATTCCGGATTGGGTTCATCC 45.0 50.0 57.5 57.6 1106 bp 1.0 73.2
petA-psbJ_p5 ACGTGTTCAAGGCCTTTTGT ATTCCGGATTGGGTTCATCC 45.0 50.0 58.5 57.6 1133 bp 1.0 73.1