Hotspot

ycf4-cemA

LSC · 63011-63912 · 902 bp

Back to Eurya

Pi 0.000665
MarkerSeek score 52.50
Primer available yes
Label ycf4-cemA

Feature Metadata

label_name
ycf4-cemA
feature_type
igs
region
LSC
length_bp
902
variable_sites
1
pi
0.000665
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
52.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf4-cemA_p1 TTGGCCTATTTCTTGCGTGT AGAGAAGAGGGGTGAATGCT 45.0 50.0 58.1 58.0 969-975 bp 1.0 79.5
ycf4-cemA_p2 TTGGCCTATTTCTTGCGTGT GAGAAATCCACCAGGGCAAA 45.0 50.0 58.1 58.1 1008-1014 bp 1.0 79.2
ycf4-cemA_p3 AAAAAGCTGCTGAATTGGCC AGAGAAGAGGGGTGAATGCT 45.0 50.0 57.8 58.0 983-989 bp 1.0 78.9
ycf4-cemA_p4 AAAAAGCTGCTGAATTGGCC GAGAAATCCACCAGGGCAAA 45.0 50.0 57.8 58.1 1022-1028 bp 1.0 78.5
ycf4-cemA_p5 AAAGCTGCTGAATTGGCCTA AGAGAAGAGGGGTGAATGCT 45.0 50.0 57.8 58.0 981-987 bp 1.0 78.5