Hotspot

rbcL-accD

LSC · 59050-59641 · 592 bp

Back to Eurya

Pi 0.00135
MarkerSeek score 48.40
Primer available yes
Label rbcL-accD

Feature Metadata

label_name
rbcL-accD
feature_type
igs
region
LSC
length_bp
592
variable_sites
2
pi
0.00135
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
48.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rbcL-accD_p1 GAGGCTAGCAAATGGAGTCC ACCCACACCTGTGTTCTAAC 55.0 50.0 58.0 57.4 743-744 bp 1.0 74.8
rbcL-accD_p2 GAGTCCTGAACTAGCTGCTG ACCCACACCTGTGTTCTAAC 55.0 50.0 57.7 57.4 729-730 bp 1.0 72.8
rbcL-accD_p3 GGCTAGCAAATGGAGTCCTG ACCCACACCTGTGTTCTAAC 55.0 50.0 58.3 57.4 741-742 bp 1.0 72.5
rbcL-accD_p4 GAGGCTAGCAAATGGAGTCC CCCACACCTGTGTTCTAACT 55.0 50.0 58.0 57.1 742-743 bp 1.0 72.5
rbcL-accD_p5 GTGAGGCTAGCAAATGGAGT ACCCACACCTGTGTTCTAAC 50.0 50.0 57.6 57.4 745-746 bp 1.0 71.9