Hotspot

ndhC-trnV-UAC

LSC · 52651-53825 · 1175 bp

Back to Eurya

Pi 0.00204
MarkerSeek score 52.30
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
1175
variable_sites
5
pi
0.00204
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
52.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 TGCCAAAATAGGAATAAGAGTTGA AGAAGGTCTACGGTTCGAGT 33.3 50.0 56.3 57.8 1280 bp 1.0 40.3
ndhC-trnV-UAC_p2 GAGTTGATATTATCAGAAATGCCCA AGAAGGTCTACGGTTCGAGT 36.0 50.0 57.2 57.8 1263 bp 1.0 40.3
ndhC-trnV-UAC_p3 AGTTGATATTATCAGAAATGCCCA AGAAGGTCTACGGTTCGAGT 33.3 50.0 56.2 57.8 1262 bp 1.0 40.3
ndhC-trnV-UAC_p4 AGAGTTGATATTATCAGAAATGCCC AGAAGGTCTACGGTTCGAGT 36.0 50.0 57.0 57.8 1264 bp 1.0 40.3
ndhC-trnV-UAC_p5 TGCCAAAATAGGAATAAGAGTTGA GAAGGTCTACGGTTCGAGTC 33.3 55.0 56.3 57.5 1279 bp 1.0 40.3