Hotspot

atpI-rps2

LSC · 16549-16756 · 208 bp

Back to Eurya

Pi 0.00577
MarkerSeek score 45.60
Primer available yes
Label atpI-rps2

Feature Metadata

label_name
atpI-rps2
feature_type
igs
region
LSC
length_bp
208
variable_sites
3
pi
0.00577
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
45.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpI-rps2_p1 ATGTTGGCCTACTTCCACAC ATTTCGATTCCAGCGAACGA 50.0 45.0 57.8 58.0 391 bp 1.0 79.0
atpI-rps2_p2 ATGTTGGCCTACTTCCACAC TTTCGATTCCAGCGAACGAT 50.0 45.0 57.8 58.0 390 bp 1.0 79.0
atpI-rps2_p3 GGCCATGGACTTGGAAACTT ATTTCGATTCCAGCGAACGA 50.0 45.0 58.4 58.0 428 bp 1.0 77.6
atpI-rps2_p4 GGCCATGGACTTGGAAACTT TTTCGATTCCAGCGAACGAT 50.0 45.0 58.4 58.0 427 bp 1.0 77.6
atpI-rps2_p5 CTTGGCCATGGACTTGGAAA TTTCGATTCCAGCGAACGAT 50.0 45.0 58.4 58.0 430 bp 1.0 77.6