Hotspot

atpH-atpI

LSC · 14693-15804 · 1112 bp

Back to Eurya

Pi 0.00126
MarkerSeek score 44.70
Primer available yes
Label atpH-atpI

Feature Metadata

label_name
atpH-atpI
feature_type
igs
region
LSC
length_bp
1112
variable_sites
3
pi
0.00126
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
44.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpH-atpI_p1 ATAACGGAAGCGGCAGAAAT TTTTTGCAACTTTAGCCGCG 45.0 45.0 58.0 58.5 1200 bp 1.0 75.8
atpH-atpI_p2 TACCTTCTACAGCTTGGCCT TTTTTGCAACTTTAGCCGCG 50.0 45.0 58.0 58.5 1283 bp 1.0 75.7
atpH-atpI_p3 TACCTTGACCAACTCCAGGT TTTTTGCAACTTTAGCCGCG 50.0 45.0 57.9 58.5 1256 bp 1.0 75.2
atpH-atpI_p4 AGCCAATCCAGCAGCAATAA TTTTTGCAACTTTAGCCGCG 45.0 45.0 57.9 58.5 1216 bp 1.0 74.9
atpH-atpI_p5 AATAACGGAAGCGGCAGAAA TTTTTGCAACTTTAGCCGCG 45.0 45.0 58.2 58.5 1201 bp 1.0 74.6