Hotspot

rps16-trnQ-UUG

LSC · 6323-8022 · 1700 bp

Back to Eurya

Pi 0.00153
MarkerSeek score 52.10
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
1700
variable_sites
5
pi
0.00153
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
52.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 AACGGATCGTGTCCTTCAAG GAAATTGAAATGGGGCGTGG 50.0 50.0 57.9 58.0 1846-1871 bp 1.0 79.7
rps16-trnQ-UUG_p2 AAGTCGCACGTTGCTTTCTA GAAATTGAAATGGGGCGTGG 45.0 50.0 58.1 58.0 1829-1854 bp 1.0 79.2
rps16-trnQ-UUG_p3 AACGGATCGTGTCCTTCAAG GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.9 58.1 1788-1813 bp 1.0 79.2
rps16-trnQ-UUG_p4 GTCGAGTCAAGAGCACCTTT GAAATTGAAATGGGGCGTGG 50.0 50.0 57.8 58.0 1900-1925 bp 1.0 79.1
rps16-trnQ-UUG_p5 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 1771-1796 bp 1.0 78.7