Hotspot

trnS-GCU

LSC · 8416-8503 · 88 bp

Back to Euphrasia

Pi 0.053

MarkerSeek score 69.50

Primer available yes

Label trnS-GCU

Feature Metadata

label_name: trnS-GCU
feature_type: tRNA
region: LSC
length_bp: 88
variable_sites: 7
pi: 0.053
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU_p1	CCCCGGACGTGAAGAATAAA	GGAACTTCCTGATTTGCGGA	50.0	50.0	57.6	58.2	286 bp	1.0	81.4
trnS-GCU_p2	AACCCCGGACGTGAAGAATA	GGAACTTCCTGATTTGCGGA	50.0	50.0	58.7	58.2	288 bp	1.0	78.7
trnS-GCU_p3	ACCCCGGACGTGAAGAATAA	GGAACTTCCTGATTTGCGGA	50.0	50.0	58.7	58.2	287 bp	1.0	78.7
trnS-GCU_p4	TAACCCCGGACGTGAAGAAT	GGAACTTCCTGATTTGCGGA	50.0	50.0	58.7	58.2	289 bp	1.0	78.7
trnS-GCU_p5	ACCCCGGACGTGAAGAATA	GGAACTTCCTGATTTGCGGA	52.6	50.0	58.0	58.2	287 bp	1.0	76.4