Hotspot

trnR-UCU

LSC · 9996-10067 · 72 bp

Back to Euphrasia

Pi 0.0556

MarkerSeek score 63.20

Primer available yes

Label trnR-UCU

Feature Metadata

label_name: trnR-UCU
feature_type: tRNA
region: LSC
length_bp: 72
variable_sites: 6
pi: 0.0556
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU_p1	ACGAAAAATCGGAATGAACG	AGCCTTTACTTGCTATCGAA	40.0	40.0	55.0	54.5	135 bp	1.0	47.6
trnR-UCU_p2	ACGAAAAATCGGAATGAACG	AGCCTTTACTTGCTATCGAAA	40.0	38.1	55.0	55.3	135 bp	1.0	47.6
trnR-UCU_p3	ACGAAAAATCGGAATGAACG	AAGCCTTTACTTGCTATCGA	40.0	40.0	55.0	54.5	136 bp	1.0	47.5
trnR-UCU_p4	ACGAAAAATCGGAATGAACG	AAAGCCTTTACTTGCTATCGA	40.0	38.1	55.0	55.3	137 bp	1.0	47.5
trnR-UCU_p5	ACGAAAAATCGGAATGAACG	AAGCCTTTACTTGCTATCGAA	40.0	38.1	55.0	55.3	136 bp	1.0	47.5