Hotspot

rps2

LSC · 15552-16262 · 711 bp

Back to Euphrasia

Pi 0.0272

MarkerSeek score 53.30

Primer available yes

Label rps2

Feature Metadata

label_name: rps2
feature_type: gene
region: LSC
length_bp: 711
variable_sites: 29
pi: 0.0272
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps2_p1	ACCGATTCCCAAAATCTATTGT	TCAGGGTTCCTCGTCTCTTT	36.4	50.0	55.8	58.0	812 bp	1.0	52.8
rps2_p2	ACCGATTCCCAAAATCTATTGT	TTCAGGGTTCCTCGTCTCTT	36.4	50.0	55.8	58.0	813 bp	1.0	52.8
rps2_p3	ACCGATTCCCAAAATCTATTGT	TTTCAGGGTTCCTCGTCTCT	36.4	50.0	55.8	58.0	814 bp	1.0	52.8
rps2_p4	ACCGATTCCCAAAATCTATTGT	GTTTCAGGGTTCCTCGTCTC	36.4	55.0	55.8	57.9	815 bp	1.0	52.3
rps2_p5	ACCGATTCCCAAAATCTATTGT	CAGTTTCAGGGTTCCTCGTC	36.4	55.0	55.8	58.2	817 bp	1.0	51.3