Hotspot

atpF

LSC · 11787-13028 · 1242 bp

Back to Euphrasia

Pi 0.0269

MarkerSeek score 61.60

Primer available yes

Label atpF

Feature Metadata

label_name: atpF
feature_type: gene
region: LSC
length_bp: 1242
variable_sites: 50
pi: 0.0269
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF_p1	CACCCGCCATTACTTCATCA	AGAGGGAAAAATACGGGGGA	50.0	50.0	58.0	58.0	1936-1939 bp	1.0	86.9
atpF_p2	ATTCGACTAATTCACCCGCC	AGAGGGAAAAATACGGGGGA	50.0	50.0	58.1	58.0	1948-1951 bp	1.0	86.8
atpF_p3	AACGCCGGGAAATAATACCC	AGAGGGAAAAATACGGGGGA	50.0	50.0	58.0	58.0	2206-2209 bp	1.0	86.6
atpF_p4	AACGCCGGGAAATAATACCC	GAGGGAAAAATACGGGGGAC	50.0	55.0	58.0	58.0	2205-2208 bp	1.0	86.4
atpF_p5	AACGCCGGGAAATAATACCC	GGGAAAAATACGGGGGACTC	50.0	55.0	58.0	58.0	2203-2206 bp	1.0	86.4